According to Business Week magazine, these are the five biggest turnoffs that get interview candidates disqualified:

1. Playing hard to get. Acting nonchalant will usually be interpreted that you aren't interested enough in the position.
2. Talking too much. Never ramble nor entertain the interviewer with chitchat about the Super Bowl.
3. Boasting. You must sell yourself, but beware of embellishing your responsibilities, exaggerating your accomplishments, covering up short­comings or bragging too much.
4. Not listening. Candidates are often so anxious to prove themselves that they don't listen carefully to the interviewer's questions. As a result, their answers are target.
5. Being unprepared. If you haven't done your homework to learn about the company and its culture, you can't ask the right questions.

Poor posture ... a limp handshake ...bow ties ... smoking a pipe ... too much jewelry... nervous tics ... nervous demeanor.

Finally, if you're a man, and you cross your legs, make sure your socks rise high enough to cover any bare skin. If they don't, do not cross your legs. Believe it or not, an exposed hairy calf ranked highest in one survey of what turns women off most.